Hello guest, Register  |  Sign In
Virologica Sinica, 01 (1) : 31, 1986
Review
IDENTIFICATION AND MOLECULAR CLONING OF DNA OF BUZURA SUPPRESSARIA NUCLEAR POLYHEDROSIS VIRUS
Wuhan Institute of Virology
Academia Sinica
Wuhan
 Correspondence:
(597.17KB)  
Abstract
The DNA of Buzura Suppressaria Nuclear Polyhedrosis Virus(BsNPV) was digested at least9 fragments by restriction endonuclease BamHI.The fragments eluted by polyacrylamide gel electrophoresis were recombined with plasmid pBR_(322)incubated with BamHI and transfered into E.coli. Antibiotic screening,electrophoresis analysis and colony hybridization showed the existence of insert sequence in hybrid plasmid.The result suggested that the cloning of BamHI fragments of BsNPV DNA be successful.
Key Words:
  Published online: 5 Jan 1986
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved