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Virologica Sinica, 01 (4) : 30, 1986
Review
ISOLATION AND PROPAGATION OF HEPATITIS A VIRUS IN VITRO
Department of Viral Disease of Control
Health and Anti-epldemic Station of Sichuan Province
Chengdu
Health and Anti-epldemic Station of Sichuan Province
Chengdu
Correspondence: 
(389.52KB)
Abstract
A Strain of hepatitis A virus(S_(84-1))was directly isolated from a fecal specimen of acute patient with hepatitis A and serially passaged in human diploid fibroblast(2BS). Some conditions of cultivation of this virus were also studied. Following serial propagation, the incubation time of S_(84-1) virus was reduced from one and half months(passage 2) to 3 days(passage 12). S_(84-1) virus, without cytopathie effect, can t be detected in supernatant fluid by ELISA and was harvested by freezing-throwing the ce...
Key Words:
Published online: 5 Dec 1986
