Department of Viral Disease of Control Health and Anti-epldemic Station of Sichuan Province Chengdu
Correspondence:
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Abstract
A Strain of hepatitis A virus(S_(84-1))was directly isolated from a fecal specimen of acute patient with hepatitis A and serially passaged in human diploid fibroblast(2BS). Some conditions of cultivation of this virus were also studied. Following serial propagation, the incubation time of S_(84-1) virus was reduced from one and half months(passage 2) to 3 days(passage 12). S_(84-1) virus, without cytopathie effect, can t be detected in supernatant fluid by ELISA and was harvested by freezing-throwing the ce...