Hello guest, Register  |  Sign In
Virologica Sinica, 01 (4) : 43, 1986
Review
THE PRODUCTION OF AN ANTI-NSV-1 LYMPHOCYTE HYBRIDOMA CELL LINE AND TYPING OF HSV ISOLATES WITH MONOCLONAL AHTIBODIES
Dept. Microbiology
The Fourth Military Medical College
Xian
 Correspondence:
(248.72KB)  
Abstract
A hybridoma cell line(Mad-2) was produced by fusing mouse myloma cell line Sp2/O with the spleen cells immunized by HSV-1 SM44 strain infected BHK-21 cells and membrane protein antigen extracted from the HSV-1 infected ceils, the positive clone Mad-2 secreating monoclonal antibody(McAb) against HSV-1 was selected by ELISA. the supernatant and ascites producet by Mad-2 all reacted with HSV-1, hut not with HSV-2. the titer of McAb in ascites mesured by ELISA was 10~(-7) and the subclass of the Mad-2 was IgG1....
Key Words:
  Published online: 5 Dec 1986
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved