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Virologica Sinica, 02 (3) : 13, 1987
Review
DETECTION OF HEPATITIS A ANTIGEN BY A CELL CULTURE FLUORESCENT ANTIBODY TECHNIQUE WITH BIOTIN-AVIDIN
National Institute for the Control of Pharmaceutical and Biological Products
Beijing
National Center for Drugs and Biologics
FDA
Bethesda
MD 20205
U. S. A.
 Correspondence:
(256.24KB)  
Abstract
This paper describes an improved fluorescent antibody technique for the detection of HAV. The assay is unique in its use of the characteristic of a highaffinity complex between fluorescence-conjugated avidin and biotin-linked anti-HAV to increase sensitivity. We have made a comparison between direct-fluorescent and fluorescentantibody technique with biotin-avidin with same lot IgG antibody and same lot ACMK inoculated HAV. Because of the high binding affinity of this system due to multiple binding of biotin...
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  Published online: 5 Sep 1987
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