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Virologica Sinica, 02 (3) : 28, 1987
Review
DETECTION OF IgM ANTI-HBc BY ENZYME LINKED IMMUNOSORBENT ASSAY WITH AVIDIN LABELLED PEROXIDASE AND BIOTIN LABELLED ANTI-HBc
Laboratory of Clinical Immunology
Tongji Hospital
Tongji Medical University
Wuhan
 Correspondence:
(360.20KB)  
Abstract
A new immunoenzymatic assay was established with avidin labelled peroxidase(A-P) and biotin labelled anti-HBc(B-Anti-HBc)for deteeting IgM antibody to hepatitis B core antigen. A-P, B-Anti-HBc and antiserum to human μ chain for the assay were prepared by our laboratory, Aggregated normal rabbit IgG was added to the buffer solution employed as a dilution to overcome the influence of rheumatoid factor of the blood specimens The specificity of the method was proved by SPA absorption method and anti-HBc inhibit...
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  Published online: 5 Sep 1987
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