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Virologica Sinica, 02 (4) : 43, 1987
Review
PILOT PRODUCTION OF HIGH-TITRE MOUSE INTERFERON WITH MICROCARRIER SUSPENSION CULTURE SYSTEM
Institute of Microbiogy and Epidemiology
Academy of Military Medical Sciences
Beijing
 Correspondence:
(677.47KB)  
Abstract
With MC-1 type microcarrier at concentration of 5mg/ml, the mouse L929 cell line could attach and proliferate on them very well if the stirring speed was lower than 50r/m.When the cell density was inoculated about 30×10~4/ml,it could proliferate to 1×10~6/ml after 3 days generally. At this time,priming with 25 IU/ml mouse IFN about 12-24 h and then induced with NDV and superinduced with cycloheximide(20μg/ml) and actinomycin D(2μg/ml),the titer of IFN in the media would be 10~5IU/ml approximately, or 10~5IU...
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  Published online: 5 Dec 1987
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