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Virologica Sinica, 03 (2) : 124, 1988
Review
Purification and Characterization of HFRS Virus 50K Structural Protein
Department of Microbiology
Fouth Military Medical College
Xian
 Correspondence:
(697.06KB)  
Abstract
A method has been developed to purify HFRS virus structural protein. HFRS virus was first purified roughly by protamine sulfate and polyethylene glycol precipitation from brain tissue of suckling mice infected with HFRS virus strain 76-118 or strain Chen, and the roughly purified virus was then passed to immunoadsorbent column prepared by Sepharose 4B coupled with McAb against HFRS virus. When the column was eluted with potassium thiocyanate a viral protein, it showed only one band on SDS-PAGE and its molec...
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  Published online: 5 Mar 1988
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