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Virologica Sinica, 03 (2) : 131, 1988
Analysis of Japanese Encephalitis Viral Structural Polypeptides by SDS-PAGE Silver Staining
National Institute for the Control of Pharmaceutical and Biological Products
Japanese encephalitis virus SA14 strain(JEV) precipitated by 9% PEG 6000 from cultural medium of C_3/36 cells has been purified by centrifugation through a 20% and 60% sucrose discontinuous gradient. The polypeptides of JEV-SA14 strain were analysed in a discontinuous SDS-PAGE silver staining system. Three structural polypeptides of JEV-SA14 strain with MW of 53,000(VP_3), 12,200(VP_2) and 9,200(VP_1) were identified respectively. These structural polypeptides presenting in the form of monomer were demonstr...
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  Published online: 5 Mar 1988
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