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Virologica Sinica, 03 (2) : 160, 1988
Proliferation of Cells and Virus with Microcarrier Suspension Culture System
Institute of Microbiology and Epidemiology
Academy of Military Medical Sciences
We have successfully cultured three cell lines BHK-13, BHK-21 and CHO-KI with MC-1 type microcarrier made in our Academy. When the microcarrier was in concentration of 5mg/ml, the cell density was about 30×10~4 cells/ml, all of them could proliferate to 1×10~6 cells/ml after 3-day suspension culture. At this time, inoculated VSV diluted 10 or 10~2 times, incubated at 37℃ for 20-24hs, the viral titer in these media were about 6 Log TCID_(50) or much higher. The yield of CHO-KI cell was the highest. Its titre...
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  Published online: 5 Mar 1988
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