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Virologica Sinica, 03 (3) : 285, 1988
Isolation of Hepatitis A Virus in Vitro Directly from Peripheral Blood Mononuclear Cell of Patients with Hepatitis A
Institute of Military Medicine
Nanjing Command
Nanjing Hospital for Infectious Disease Nanjing
81th Hospital
Nanjing Command.PLA
HAAg were detected from the samples of peripheral blood mononuelear leukocytesof patients with hepatitis A using IF technique. The positive samples weer frozen and thawed 3 times and inoculated into PLC/PRF/5 cell cultures. Two strains of HAV were isolated, The isolates proved to be HAV based on specific activity in serologic tests and morphology. The results of our studies suggested that HAV be present not only in the serum freely but also associated with leukocytes. It is a simple method to screen the sam...
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  Published online: 5 Sep 1988
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