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Virologica Sinica, 03 (3) : 293, 1988
Studies on the Homology of Attacus ricini Nuclear Polyhedrosis Virus and Polyhedrin Gene of Autographa californica Nuclear Polyhedrosis Virus
Dept.of Virology.Wvhan University
The recombinant DNA (PMA-VI DNA) containing cDNA of mRNA of polyhedringene of Autographa californica nuclear polyhedrosis virus(AcNPV) was used to transform E.coli RR_1, and colonies were selected by antibiotic resistance and colony hybridization.The colony containing PMA-VI plasmid grew in L.Broth.The plasmid DNA was rapidly isolated by boiling method, and tben recovered by electroelution. With the plasmid DNA as a probe and by Southern hybridization, the results showed that the sequence homologues to the ...
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  Published online: 5 Sep 1988
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