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Virologica Sinica, 03 (4) : 357, 1988
Studies on WMV-2 aud CMV Detection Using Protein A Sandwich ELISA Method
Nanjing Agricultural University
Dept. of Plant Protection
A new form of indirect enzyme-linked immunosorbent assay (ELISA) has been used for detection of WMV-2 and CMV in watermelon plants. The method uses protein A in two applications to direct ELISA method. The first layer of protein A is a precoating layer for the coating antibody. The second layer of protein A is conjugated enzyme for detecting the second antibody layer. In practical application, Protein A sandwich ELISA (PAS-ELISA) was more sensitive and specific than the direct double antibody sandwich form ...
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  Published online: 5 Dec 1988
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