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Virologica Sinica, 03 (4) : 364, 1988
Preparation and Immunological Properties of Monoclonal Antibodies to Ribgrass Mosaic Virus (RMVsh)
Department of Biology
Fudan University
Using hybridoma techniques, seven hybridoma cell lines: 1H2, 7H1, 10H1, 11H2, 12H3, 17H6, 29H1, secreting monoclonal antibodies to Shanghai Isolate of Ribgrass Mosaic Virus(RMVsh)were prepared by fusion on myeloma cells SP2/O with spleen cells of BALB/c mouse immunized with RMVsh. The subclasses of these monoclonal antibodies were determinated by double antibody sandwich ELISA. 1H2, 7H1 and other monoclonal antibodies belong to IgG_3, IgG_(2b) and IgG_1 subclass, respectively. The ascitic fluids were obtain...
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  Published online: 5 Dec 1988
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