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Virologica Sinica, 03 (4) : 370, 1988
An Effecive procedure for the Separation and Preparation of Citrus Exocortis Viroid
Wuhan Institute of Virology
Academia Sinica
Viroid is small and exists less in host, which is difficult to purify. General method of purification is phenol extract, remove carbohydrate, dialysis, LiCl partition, DNase degradation and cellulose chromatography. The procedure is complicated, recovery ratio is less and the pure sample which is adapted to all kinds of use can t be gotten. A procedure for the separation and preparation of citrus exocortis viroid (CEV)is reported Nucleic acids were extracted from citrus leaves by phenolchloroform treament T...
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  Published online: 5 Dec 1988
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