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Virologica Sinica, 04 (1) : 28, 1989
Preparation and Application of the Monoclonal Hybridoma Antibodies against the Epidemic Hemorrhagic Fever Virus (EHFV) in Anhui Province
Division of Virclogy
Anhui Institute of Medical Sciences
Two monoclonal antibodies(McAb) were obtained by fusion of SP2/O myeloma cells with spleen cells from Balb/c mice. They were respectively immunized with the Li strain and the hemagglutinin(HAN) from the Chen strain of EHFV. Two fusions had been done. The growth rate of hybridoma was 97.5% and 60%. The positive rate was 2.5% and 22%. The positive rate of cloning all achieved 100%. Finally two hybridoma cell lines had been established. They were separately designated as 2B_7 and 1H_8. The titers of two ascite...
  Published online: 5 Mar 1989
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