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Virologica Sinica, 04 (1) : 28, 1989
Review
Preparation and Application of the Monoclonal Hybridoma Antibodies against the Epidemic Hemorrhagic Fever Virus (EHFV) in Anhui Province
Division of Virclogy
Anhui Institute of Medical Sciences
Hefei
 Correspondence:
(156.42KB)  
Abstract
Two monoclonal antibodies(McAb) were obtained by fusion of SP2/O myeloma cells with spleen cells from Balb/c mice. They were respectively immunized with the Li strain and the hemagglutinin(HAN) from the Chen strain of EHFV. Two fusions had been done. The growth rate of hybridoma was 97.5% and 60%. The positive rate was 2.5% and 22%. The positive rate of cloning all achieved 100%. Finally two hybridoma cell lines had been established. They were separately designated as 2B_7 and 1H_8. The titers of two ascite...
  Published online: 5 Mar 1989
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