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Virologica Sinica, 04 (1) : 100, 1989
Review
Use of AntibOdy Sensitized Human O Red Blood Cells for Reverse Passive Hemagglutination Test(RPHA) and RPHA Inhibition Test(RPHI) for Detection of Antigen and Antibody
National Institute for the Control of Pharmaceutical and Biological Products
Beijing
 Correspondence:
(136.86KB)  
Abstract
We have studied RPHA and RPHI test using anti-EHFV antibody sensitized human red blood cell for detecting EHF antigen and antibody. The antigen used in RPHI was successfully prepared with infected mouse brains extracted by pH 9.0 borate buffered saline(BBS), providing a method of simplicity for preparing antigen. This RPHA test can be used for detection on antigen content of EHF vaccine instead of ELISA, and for detection of EHF virus in tissue culture fluid instead of using IF technique for detecting EHF a...
  Published online: 5 Mar 1989
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