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Virologica Sinica, 04 (2) : 172, 1989
Review
Preparation of Monoclonal Antibodies to Papaya Ringspot Virus and Application in Serotyping
1.South China Agricultural University
Guangzhou.
2. The Frist Medicine University of PLA
 Correspondence:
(121.66KB)  
Abstract
Three stable hybridoma cell lines secreting monoclonal antibodies against papaya ringspot virus were produced by fusing spleen cells of mice immunized with papaya ringspot virus(PRV-1) to mouse myeloma cell lines Sp2/0-Ag14. of three hybridoma cell lines, the titer of 23H1 secreting monoclonal antibody was found to be the highest, when testing by ELISA method, the titer of 23H1 inascitie fluid was 1:76800. Three cell lines secreting monoclonal antibodies were specific for papaya ringspot virus, not reactive...
  Published online: 5 Jun 1989
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