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Virologica Sinica, 04 (2) : 172, 1989
Preparation of Monoclonal Antibodies to Papaya Ringspot Virus and Application in Serotyping
1.South China Agricultural University
2. The Frist Medicine University of PLA
Three stable hybridoma cell lines secreting monoclonal antibodies against papaya ringspot virus were produced by fusing spleen cells of mice immunized with papaya ringspot virus(PRV-1) to mouse myeloma cell lines Sp2/0-Ag14. of three hybridoma cell lines, the titer of 23H1 secreting monoclonal antibody was found to be the highest, when testing by ELISA method, the titer of 23H1 inascitie fluid was 1:76800. Three cell lines secreting monoclonal antibodies were specific for papaya ringspot virus, not reactive...
  Published online: 5 Jun 1989
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