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Virologica Sinica, 04 (2) : 208, 1989
Cytomegalovirus Neutral Red Plaque Assay And High Titer Virus Preparation
Department of Microbiology
Shanghai Medical University
A neutral red plaque assay was established for the quantitation of infections cytomegalovirus(CMV), The plaque counts linearly correlated to the virus dilutions, indicating the accuracy of this method. Using of the conventional reagents and instruments and quicker results (8 days)may facilitate the application of the assay, With this assay, it was found that rotating culture of CMV-infected cells could easily produce infectious CMV titer as high as 1.8×10~7 PFU/ml, when the culture medium supernatant was co...
  Published online: 5 Jun 1989
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