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Virologica Sinica, 04 (3) : 245, 1989
Review
Cloning and ldentification of the Type-specific DNA Fragment of Herpes Simplex Virus Type 2
Dept. of Microbiology
Xi-an Medical University
Xi-an
 Correspondence:
(168.07KB)  
Abstract
The DNA of Herpes Simplex Virus type 2 (HSV-2)digested with restrictionenzyme BamHI and the BamHI G fragment was isolated, which locates in thereverse repeat sequence and spans the junction site of L and S component.Then the BamHI G was inserted on the BamHI cut-site of the vector plasmidpUC 8. In order to modify this recombinant plasmid, the EcoRI--KPN I fra-gment of the recombinant plasmid was removed by restriction enzyme digestionwith EcoRI and KPN 1. After modification of the ends and ligation of them,...
  Published online: 5 Sep 1989
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