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Virologica Sinica, 04 (4) : 350, 1989
The Purifications of Mouse Ascitic Tumor Virus(SRSV) and Viral Ribonucleic Acid(SRSV RNA)and Investigation of Some Properties
Department of Biophysics and Pathophysiology
Shanghai Medical University
SRSV was obtained from the supernatant of infected NIH 3T3 mouse fiblasts and purified by sucrose density gradient centrif ugation.SRSV RNA was prepared from fresh viral pellets and extracted with chloroform-phen(?)l and fractionated on sucrose gradients. The RNAs of various fractions were determined by sedimeptation coefficient(s) and followed by gel electrophoresis. The component of SRSV RNA contains two main fractions, one sedimenting at about 60-70s(after treatment with heat at about 35S), plus small am...
  Published online: 5 Dec 1989
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