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Virologica Sinica, 04 (4) : 365, 1989
Detection of Dengue Virus RNA by DNA-RNA Dot-spot Hybridization
Department of Microbiology and Immunology
Sun Yat-sen University of Medical Sciences
Biotechnology Research Center
Zhongshan University
Recently we have prepared large amount of purified pEH 920 DNA which is inserted by complementary DNA of dengue virus 2 RNA. Using the [a-~(32)p] dCTP labelled pEH 920 DNA as the probe, dengue virus RNA in the supernatants of infected C6/36 mosquito cells have been detected by DNA-RNA dot-spot hybridization. The results show that the probe strongly reacted with dengue 2 virus RNA though it also cross-hybridized with dengue 1, 3 and 4 virus RNA. The preliminary experiments show that the probe can at least de...
  Published online: 5 Dec 1989
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