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Virologica Sinica, 04 (4) : 427, 1989
The cDNA of Assembly Origin Sequence in TMV Genome Inhibiting TMV Assembly in Vitro
Shanghai Institute of Biochemistry
Academia Sinica. Shanghai
A 20 nucleotides DNA fragment which is complementary with the assembly origin of TMV-RNA was synthesized. Instead of antisense RNA, the DNA fragment could hybridize with TMV-RNA in nitrocellulose dot hybridization and in mini-volume solution hybridization assays. Using RNA-cDNA complex and 20S aggregates of TMV coat protein, the TMV in vitro assembly reaction experiment, which was monitored by 310nm absorption spectrum and electron microscopy, showed that TMV in vitro assembly could not proceed in results o...
  Published online: 5 Dec 1989
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