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Virologica Sinica, 05 (1) : 36, 1990
Detection of the Antigen of Human Cytomegalovirus Isolates in Cell Culture by Indirect Immunofluorescent Assay Using Monoclonai Antibody
Department of Microbiology
Tongji Medical University
Wuhan 430030 Wuhan Institutc of Virology
Academia Sinica
Wuhan 430071
Two indirect immunofluorescent assays(IIFA), using a monoclonal antibody directed against protein antigen (McAb-20k)and specific positive sera of human cytomegalovirus(HCMV)respectively, were applied for the rapid detection of the virus from fifty urine specimens(28 cases)inoculated onto human embryonic fibroblast. By the former procedure g of 28 cases were positive at 48 hours postinfection and all of them developed cytopathic effect; by the latter 11 were positive at 6 days postinfection and only 10 cases...
  Published online: 5 Mar 1990
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