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Virologica Sinica, 05 (1) : 41, 1990
Development of Method for the Detection of Enteroviruses from Clams
School of Public Health
Shanghai Medical University
Shanghai 200032
A procedure for concentrating and recovering enteroviruses from clams (Arca( Anadara)subcrenata Lischke)has been developed and evaluated. Viruses were efficiently adsorbed to homogenized clam tissue by adjusting the homogenate to pH4.5. After low-speed centrifugation, the viruses were eluted from the sediment with pH 9.5 0.05mol/L glycine-saline. Instead of using Cat-Floc in Johnson s method, AlCl_3 was used to remove cytotoxity from clam tissue homogenates. After low-speed centrifugation, viruses were conc...
  Published online: 5 Mar 1990
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