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Virologica Sinica, 05 (2) : 193, 1990
Purification, Serology and Other Properties of Wheat Yellow Mosaic Virus(WYMV)
Virology Laboratory
Zhejiang Academy of Agricultural Sciences
Wheat yellow mosaic virus(WYMV) has been occurred on wheat in Shanxi province since 1970s. The virus preparation was purified from naturally infected wheat plants by extracting in 0.5mol/L potassium phosphate buffer pH7.0, clarifying in 1/4 v carbon tetrachloride, precipitating with 6% PEG, 3% NaCl and 1% Triton X-100 followed by two cycles of differential centrifugations with 20% sucrose cushions containing 0.3% Triton X-100 and finally centrifuged in 10-50% sucrose density gradients. The yields of virus p...
  Published online: 5 Jun 1990
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