Hello guest, Register  |  Sign In
Virologica Sinica, 05 (2) : 193, 1990
Review
Purification, Serology and Other Properties of Wheat Yellow Mosaic Virus(WYMV)
Virology Laboratory
Zhejiang Academy of Agricultural Sciences
Hangzhou
310021
 Correspondence:
(214.08KB)  
Abstract
Wheat yellow mosaic virus(WYMV) has been occurred on wheat in Shanxi province since 1970s. The virus preparation was purified from naturally infected wheat plants by extracting in 0.5mol/L potassium phosphate buffer pH7.0, clarifying in 1/4 v carbon tetrachloride, precipitating with 6% PEG, 3% NaCl and 1% Triton X-100 followed by two cycles of differential centrifugations with 20% sucrose cushions containing 0.3% Triton X-100 and finally centrifuged in 10-50% sucrose density gradients. The yields of virus p...
  Published online: 5 Jun 1990
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved