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Virologica Sinica, 05 (2) : 221, 1990
Selection of Super Reproductive Parvovirus Strains for Vaccine Preparation by Alternate Cultivation in Cell Lines with Very Different Susceptibiilty
Economic Animal Virology and Immunology Laboratory
Military Veterinary Science Institute in Beijing Military Area of PLA. Beijing 100071
This study has succeeded in selecting two strains(S_(18), S_(36)) of MEV with reproductive titre up to HA8192~×-32768~× or TCID_(50) Log_(10)~.7-10.3) from 6 strains of MEV with the titre 2~×- 64~× by alternate cultivation of 22 passages in two feline kidney cell(FKC) lines of susceptibility F_(81) strain and insusceptibility C_(81) strain ever reported as yet. The systematic tests proved that SMEV_(18,36) strains selected at present by alternative cultivation are super reproductive with powerful and extens...
  Published online: 5 Jun 1990
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