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Virologica Sinica, 05 (3) : 312, 1990
The Purification of Citrus Tristeza Virus
Fujian Academy of Agricultural Sciences
Fushou 350003
This paper deals with the Purification method of citrus tristeza virus (CTV) from infected sweet orange(Citrus sinensis[L]. Osbeck). The frozen and infected tissues were homogenized with 0.1mol/L Tris-buffer pH8.4 which contains 0.15% TritonX-100. After several differential centrifugations including two times of precipitation with PEG (MW 6,000), the supernatant was centrifuged by incontinuous sucrose density gradient of 200—400mg/ml and 600 mg/ml at 30,000 r/m (Hitachi, RPT 40 rot) for 3 hours. Then the ba...
  Published online: 5 Sep 1990
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