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Virologica Sinica, 05 (3) : 312, 1990
Review
The Purification of Citrus Tristeza Virus
Fujian Academy of Agricultural Sciences
Fushou 350003
 Correspondence:
(128.43KB)  
Abstract
This paper deals with the Purification method of citrus tristeza virus (CTV) from infected sweet orange(Citrus sinensis[L]. Osbeck). The frozen and infected tissues were homogenized with 0.1mol/L Tris-buffer pH8.4 which contains 0.15% TritonX-100. After several differential centrifugations including two times of precipitation with PEG (MW 6,000), the supernatant was centrifuged by incontinuous sucrose density gradient of 200—400mg/ml and 600 mg/ml at 30,000 r/m (Hitachi, RPT 40 rot) for 3 hours. Then the ba...
  Published online: 5 Sep 1990
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