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Virologica Sinica, 05 (3) : 317, 1990
Detection of Plant Viruses by Dot-immunobinding Assay
Section of Plant Pathology
Nanjimg Agricultural University
Nanjing 210014
TuMV, SMV, and CMV were detected by dot-immunobinding assay (DIA) at dilutions of 1:5120, 1:2560 and 1:1280, respectively. DIA successfully detected 1.7ng of purified SMV and 1.2ng of purified CMV. DIA was used with horseradish peroxidase-linked goat anti-rabbit IgG and nitrocellulose membrane was used as the solid phase. 3, 3-Diamino-benzidine was used as the suhstrate and got good results. Absorption of the first antibody with healthy plant sap was necessary to eliminate background color. This assay is si...
  Published online: 5 Sep 1990
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