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Virologica Sinica, 05 (4) : 341, 1990
Review
Studies on Semimicro Quick Monolayer Plaque Assay of Viruses
Department of Microbiology
Nanjing Railway Medical College
Nanjing
210009Department of Microbiology
Zhejiang Medical University
Hangzhou
PRC. 310006
 Correspondence:
(173.73KB)  
Abstract
A semimicro quick monolayer plaque assay of viruses (SQMPAV) without preparing the monolayer in advance was developed. In this assay, the cells and viruses were simultaneously inoculated into the wells of multi-well tissue culture plates. So the attachment of cells and the adsorption of viruses occurred at the same time. Then, the plaques were produced as the monolayers were formed under the nutrient gel overlay. With the model of polioviruses, a series of studies on SQMPAV, including the experimental condi...
  Published online: 5 Dec 1990
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