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Virologica Sinica, 05 (4) : 357, 1990
Review
Development of EHF Virus Semi-micro Methylcellulose Plaque Assay
National Institute for the Control of Pharmaceutical and Biological Products
Beijing 100050
 Correspondence:
(1362.55KB)  
Abstract
A new semi-micro plaque assay has been established for epidemic hemorrhagic fever (EHF)virus, using methvlcellulose as overlay tested on Vero-E_(?) and Vero cell cultures. All EHF virus strains, including six tested on Vero-E_(?) cells and three tested on Vero cells, could form clear plaques that could be neutralized by rabbit anti-EHF virus immune serum. The results also show that the sensitivity of this method is consistent with the method of semi-micro agarose Plaque assay previously reported. However, t...
  Published online: 5 Dec 1990
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