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Virologica Sinica, 05 (4) : 404, 1990
Studies on the Methods of Preparation of ~(125)l-Labeling Viral DNA Probes
Wuhan Institute of Virology
Academia Sinica
Wuhan 430071 Jiangxi Medical college
Nachang 330006
By using BsNPV DNA, HSV DNA and λDNA, the paper reported the re suits of the influence of ionic strength, pH value, reaction temperature and time on the labeling process of DNA with radioactive iodine in the presence of thallic chloride.When pH value was 4.8—4.9, ionic strength(Na~+)was 0.1mol/L, the temperature was 70℃ and the reaction time was 30 minutes, very high specific activity can be yielded. The percentage of ~(12)Ⅰ bound to DNA is 10—30% and the specific activity of DNA probe is over 10~8 cpm/μg D...
Key Words: ~(125)I-NaI;  BsNPV;  DNA;  HSV;  λDNA;  Probe
  Published online: 5 Dec 1990
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