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Virologica Sinica, 05 (4) : 423, 1990
Review
Molecular Cloning of Bovine Herpesvirus Type 4 (DN-599 Strain) DNA
Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences
Harbin 150001USDA-ARS
Washington State University
WA 99164-7030
 Correspondence:
(217.32KB)  
Abstract
Bovine herpesvirus type 4 (BHV-4) DNA was extracted from infected MDBK (Bovine kidney) cells using Triton Ⅹ-100 and phenol/chloroform/ether. With shotgun and selective cloning procedures, the viral DNA was cloned into EcoR I, BamH I or Hind III sites of plasmids pBR322 or pUC9 after digestion of the DNA with the three restriction endonucleases, respectively, and three DNA libraries containing different restricted fragments were constructed. Photobiotin-labeled viral DNA and bovine thymus DNA were used as pr...
  Published online: 5 Dec 1990
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