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Virologica Sinica, 05 (4) : 430, 1990
Preperation and Application of Monoclonal Antibodies to Bovine Rotavirus
Institute of Animal Husbandry and Veterinary Medicine
Jiangsu Academy of Agricultural Sciences
Nanjing 210014Department of Microbiology
Nanjing General Hospital
Nanjing Corps 210014
Two monoclonal antibodies (McAb, 2C7 and 3C9) were obtained by fusion of SP 2/0 myeloma cells with spleen cells from Balb/c mice immunized with rotavirus (RV)of HN-7 strain isolated from diarrheal calf in Henan Province. All the two McAbs reacted with the common group antigen of A-group RV, because the titers of two ascite McAbs were up to 1: 10~5 or above by indirect ELISA with RV of Bovine HN-7, BRV007, BRV014 and NCDV, Porcine Na86, Simian SA-11 and Human Wa strain, but were≤ 1: 8 and≤1: 100 respectively...
  Published online: 5 Dec 1990
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