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Virologica Sinica, 06 (1) : 49, 1991
Identification of Baculovirus by Dot Hybridization
Wuhan Institute of Virology
Academia Sinica
Wuhan 430071
Present address: Northeast Agricultural College of China
Harbin 150030.
The polyhedrin gene of Prodenia lituea nuclear polyhedrosis virus(PlNPV) was mapped on the PlNPV DNA HindⅢ-A fragment by Southern-blot hybridization with the AcNPV DNA EeoRI-Ⅰ fragment as probe under 35℃ hybridization conditions. Then, two probes were prepared from PINPV DNA HindⅢ-A and PINPV DNA HindⅢ-C fragments respectively for the identification of PINPV DNA, MbNPV DNA, BsNPV DNA under different hybridization conditions. The results showed that the probe containing the polyhedrin gene was more extensive...
  Published online: 5 Mar 1991
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