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Virologica Sinica, 06 (2) : 132, 1991
Preparation of Alkaline Phosphatase Labeled HBV Probe and Its Application in Serum Detection
Wuhan Institute of Virology
Academia Sinica
Wuhan 430071
A method using nonradioactive material—alkaline phosphatase to label HBV DNA as probe has been studied and used in clinical experiments to detect the HBV DNA in hepatitis patient s serum. Alkaline phosphatase coupled with PEI (polyethyleneimine) using P-benzoquine as cross-linking reagent. The modified phosphatase was covalently linked to single stranded DNA using glutral dehyde. Such single stranded DNA enzyme complexes have been tested for blot hybridization, after hybridization and incubation with a subs...
  Published online: 5 Jun 1991
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