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Virologica Sinica, 06 (3) : 242, 1991
Review
cDNA Synthesis of BVDV RNA and the Research of Its Hybridization
Wuhan Institute of Virology
Academia Sinica
Wuhan 430071 Hunan Agricultural College
Changsha
410128
 Correspondence:
(167.34KB)  
Abstract
The BVDV(Bovine viral diarrhea virus)RNA is ssRNA.After the virus was purified, the BVDV RNA was extracted hy phenol-chloroform-isoamyl aleohl. The DNA contamination in the extracted BVDV RNA was degraded by DNase l. BVDV RNA was further purified by oligo dTcellulose affinity chromotography and agarose gel eleetrophoresis The purified BVDV RNA was polyadenylated at the 3′end under the catalysis of poly A polymerase, eDNA was synthesized with reverse transcriptase using the poly A-tailed BVDV RNA as template...
  Published online: 5 Sep 1991
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