Detection of Epidemic Hemorrhagic Fever Virus (Hantanvirus) RNA Genome with Biotin-Labeled Probe by Dot Hybridization

YANG Tie-Qiu; LI Zhi-Hong; YANG Beng; LIU Wei-Qian; DIAO Wen-Xian; LI De-Xin; HANG Chang-Shou

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Virologica Sinica, 06 (4) : 320, 1991

Review

Detection of Epidemic Hemorrhagic Fever Virus (Hantanvirus) RNA Genome with Biotin-Labeled Probe by Dot Hybridization

YANG Tie-Qiu, LI Zhi-Hong, YANG Beng, LIU Wei-Qian, DIAO Wen-Xian, LI De-Xin, HANG Chang-Shou

Virus Research Institute
Hubei Medical College
Wuhan
430071

Correspondence:

(129.28KB)

Abstract

Dot hybridization assay was developed to detect epidemic hemorrhagic fever virus(EHFV)RNA genome using probe which biotin-7-dATP labeled R-3 cDNA clone of M fragement of EHFV R-22 strain. This probe was used to detect RNA genome of the human original EHFV H-114 and H-435 strains in cell cultures. The positive hybridization signal was obtained from cells infected EHFV H-114 and H-435 strains, 5 pg cDNA or RNA could be obtained This probe do not react with DNA of cells infected herpes simplex virus and cytome...

Key Words: Biotin Hantanvirus RNA genome Dot hybridization

Published online: 5 Dec 1991