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Virologica Sinica, 06 (4) : 324, 1991
Detection of HIV-1 DNA by PCR
Institute of Microbiology and Epidemiology
A single pair of oligonucleotide primers within the envelope gene of HIV1(primer 1: 5 -AGCAGCAGGAAGCACTATGGGC-3 ; primer 2: 5 -CCAGACTGAGTTGCAACA-3 ), 142bp apart, were synthesized and used to amplify a specific fragment of the HIV-1 DNA in infected cell cultures(MT4 HIV 1), and in plasmid pⅢexE7 carrying HIV-1 envelope gene by means of polymerase chain reaction(PCR). Amplified product was identified as 142bp fragment by agrose electrophoresis and cut into a 50bp and a 92bp fragment with restriction endonuc...
  Published online: 5 Dec 1991
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