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Virologica Sinica, 06 (4) : 344, 1991
Studies on the Antisera to Narcissus Viruses
Lab. of Plant Virology
Fujian Agricultural College
Narcissus mosaic virus(NMV) was obtained from the mixed infected narcissus plants and purified from the inoculated leaves of Chenopodium amarnticolor. The procedures of purification involved precipitation with 6% PEG ( M. W. 6000 ), differential centrifugation and 20—50% sucrose density-gradient centrifugation. After 6 times of immune injection to rabbit with the purified preparation of NMV, an antiserum was prepared with a titre of 1:5000 tested by precipitation reactions in capillary pipettes about 4 week...
  Published online: 5 Dec 1991
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