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Virologica Sinica, 06 (4) : 350, 1991
Preparation of Monoclonal Antibodies to Potato Leafroll Virus
Department of Biology
Inner Mongolia University
Huhehaote 010021
The hybridoma cell lines secreting monoclonal antibodies to Potato Leafroll Virus (PLRV) were established by fusion of myeloma cells SP2/0 with spleen cells of BALB/C mouse immunized with purified PLRV by direct spleen injection followed by two tail injections. The hybridoma were screened byDot-ELISA and indirect haemagglutination test. The subclasses of monoclonal antibodies obtaied belong to IgG 2a with light chain examined by microslide gel double diffusion test. The ascitic fluid with high titer of mono...
  Published online: 5 Dec 1991
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