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Virologica Sinica, 06 (4) : 365, 1991
Study on the Purification Techniques for Strawberry Pseudo-Mild Yellow Edge Virus
Shenyang Agricultural University
Shenyang 110161
The isolated strawberry pseudo-mild yellow edge virus(SPMYEV)was maintained in the strawberry-Fragaria vesca L.var. UC-4, and was successfully extracted and purified through homogenization by polyethyleneglycol (PEG) precipitation, differential centrifugation in the sucrose cushion and continuous suerose density gradient centrifugation. The purified preparations cotained flexous rod-shaped virus particles mainly 650nm in length and 12—13nm in width. The virus showed a typitical UV spectrum of nueleoprotein ...
  Published online: 5 Dec 1991
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