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Virologica Sinica, 07 (1) : 63, 1992
Review
Identification of PINPV--DNA Recombinant Colonies with Sandwich Hybridization
Wuhan Institute of Virology
Academia Sinica
Wuhan 430071
 Correspondence:
(191.91KB)  
Abstract
The genome of the bacularvirus PINPV was digested completely into sevenfragment (A、B、C、D_1、D_2、E、F) with Hind Ⅲ. These fragments were ligated in vitro with vector pUC18 seperately and used to transform host strainE. coli TG_1. Recombinant colonies were identified with sandwich hybridization.Seven kinds of recombinant colonies containing the PINPV-DNA Hind Ⅲ fra-gments were obtained, but unfortunately the A fragment was less than theoriginal one.
  Published online: 5 Mar 1992
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