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Virologica Sinica, 07 (1) : 85, 1992
Review
Localization and Cloning of the Polyhedrin Gene of Buzura suppressaria Nuclear Polyhedrosis Virus
Wuhan Institute of Virology
Academia Sinica
 Correspondence:
(236.77KB)  
Abstract
The polyhedrin gene of tung-oil geometrid, Buzura suppressaria,nuclear polyhedrosis virus(BsNPV) was localized on BamHI-B, BglI-A, Bgl Ⅱ-F, EcoRI-P, HindⅢ-A, KpnI-I, PstI-D, XbaI-A or B and XhoI-F/G fra-gments under low stringence(40% formamide, 35℃), using the recombinantplasmid of AcNPV DNA EcoRI-I as the probe. For sequencing the polyhedringene, the KpnI-I fragment was cloned into M13mp18 vector.
  Published online: 5 Mar 1992
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