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Virologica Sinica, 07 (1) : 93, 1992
Purification and Partial characterization of Maize Dwarf Mosaic Virus
Institute of plant protection
Beijing 100094
Purified virus preparation of Maize Dwarf Mosaic Virus (MDMV) withhigh purity and infectivity was obtained by an improved procedure of puri-fication. The yield of purified virus was 0.7--1.2mg/100g infected tissue. Thevirus preparation had a typical ultraviolet absorption spectra of a filamentousvirus containing about 6% nucleic acid, A280/A260 was 0.63--0.80, Amax/Amin was 1.15. The particle size of purified virus was 720--750×14nm and themolecular weight of its coat protein was 36kd by SDS-PAGE. Antiserum...
Key Words: MDMV;  Purification
  Published online: 5 Mar 1992
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