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Virologica Sinica, 07 (1) : 106, 1992
Review
Expression of Marek s Disease Virus pp 38 Gene in Insect Cells with Use of Baculovirus as a Vector
Jiangsu Agricultural College
Yangzhou
225001 Avian Disease and Oncology
Laboratory
USDA
3606 East Mt. Hope Road
East Lansing
Michigan 48823
USA
 Correspondence:
(212.86KB)  
Abstract
The complete encoding sequence with the initiation and stop codons ofMarek s disease virus(MDV) 38 kd phosphorylated protein (pp38) gene wasintegrated into the baculovirus AcNPV transfer vector pVL1392. The insectcell line Sf9 cells were cotransfected with the recombinant transfer vectorpVLpp38 I and the wild type AcNPV DNA. A recombinant baculovirus cloneBP38 I, which was expressing the MDV pp38 gene, was screened with theanti-MDV monoclonal antibody (MAb) H19 in fluorescence antibody test.Immunoblot indic...
  Published online: 5 Mar 1992
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