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Virologica Sinica, 07 (2) : 160, 1992
Use of Digoxigenin Labelled RNA Probe in Hepatitis D Virus RNA Hybridization
1 Hebei Provincial Health & Antiepidemic Station
Baoding 071000 2. Institute of Virology
Chinese Academy of Proventive Medicine
Beijing.3. Macfarlane Burnct Centre for Medical Research
Fairfield Hospital
Vic. 3078
A RNA hyhridization assay for hepatitis D virus in serum and liver specimens, from human and infected chimpanzee experimentally, was described using a nonradioactive Digoxigenin labelled RNA probe, and was compared with the ~(32)P labelled RNA probe. Findings show that the sensitivity and specificity of the two probes are the same(about 0.2pg of homologous DNA). The results indicated that Digoxigenin labelled RNA probe can be used for efficacious detection of HDV RNA in serum and liver tissue from human or ...
  Published online: 5 Jun 1992
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