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Virologica Sinica, 07 (2) : 166, 1992
Biotinylated Lectin Blotting Analysis of Dengue-2 Virus Glycoproteins
Department of Microbiology and Immunology
Sun Yat-sen University of Medical Sciences
Guangzhou 510089
In this study we developed a method for characterization of the leetin binding properties of D2V by a panal of lectins with different sugar specificity. The dengue-2 virus(D2V) (New Guinea C strain) was cultured in Aedes alhopietus C6/36 cells. The virus-speeific and cellular proteins of the infected and mock-infected cell lysates were separated by diseontinous SDS-PAGE and transferred electrophoretically onto NC sheet. The blots were then probed with 12 kinds of biotinylated leetius(Con A, PSA, LCA, SBA, D...
  Published online: 5 Jun 1992
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