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Virologica Sinica, 07 (3) : 253, 1992
Review
Clone and Expression of Human Papillomavirus Type 16 L_1 Open Reading Frame
Department of Microbiology
Shandong Medical Univerity
Jinan
250012
Fred Hutchinson Cancer Research Center
Seattle WA 98104 USA
 Correspondence:
(146.29KB)  
Abstract
人乳头瘤病毒16型(HPV16)含有两个晚期开放读码框(L_1ORF和L_2ORF),L_1ORF编码主要衣壳蛋白,我们用质粒pHPV16、p16L_2BX_5和pATH,采用基因重组技术,制备了含有HPV16个长L_1ORF序列的基因克隆p16L_1BN(5071—253),它能在大肠杆菌中有效表达,产生分子量约90KD的含有E. coli trpE的融合蛋白。Western blot检测,该蛋白可被抗牛乳头瘤病毒的抗体识别,这说明克隆p16L_1BN能有效表达,基因产物具有乳头瘤病毒型的共同抗原的性质。
  Published online: 5 Sep 1992
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