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Virologica Sinica, 07 (3) : 304, 1992
New Technique for the Production of Viral Insecticide of B. suppressaria Nuclear Polyhedrosis Virus
Wuhan Institute of Virology
Academia Sinica
Wuhan 430071
Pu-qi Institute of Tea Sciences in the Farm
Puqi county
The oil tree moth (B. suppressaria larvae) was grown to 4 instar using a seml-synthetic artificial diet. then infected with (BsNPV) inoculum propagated in the insect cell cultures. 7 days p.i., the virus-infected larvae were collected and virus (PIB) was purified by differential centrifugation. Then, a flowable formulation was made with the glycerin, virus-protective agent, emulsion and antibiotic preparation was determined by standard bioassays using laboratory-reared larvae on artificial diet. LC50 was 2....
  Published online: 5 Sep 1992
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