Wuhan lnstitute of Virology Academia Sinica Wuhan 430071
Correspondence:
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Abstract
In this paper four different strains of RHDV were purified by two phases of polyethylene glyeol-dextran sodium sulfate andcentrifugation, then virions in highly pure form were obtained by using Sepharose 4B chromatography. The recovery rate of virus preparation was about 70 pereent. Serological relationships of four different strains of RHDV were studied by using immuno double diffusion, cross hemagglutination inhibition and enzyme-linked immunosorbent assay(ELISA). The results showed that serotypes are the...